Evaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model

Abstract A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation soft tissue regenerative possibilities after subcutaneous implantation biomaterials – silver/poly(vinyl alcohol) (Ag/PVA) and novel alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to instructions international ISO 10993-6, 2007. Histological sections observed 7, 15, 30 60 days grafting. These hydrogels by situ electrochemical synthesis silver nanoparticles polymer matrices, enabled obtaining completely safe biocompatible materials, free from any additional toxic chemical reducing agents. Surgical was done permission Ethical Committee Faculty Veterinary Medicine, University Belgrade. Immunohistochemical (IHC) studies included assessment smooth muscle expression actin blood vessels (α-SMA), laminin type I III collagen skin structures, and, determination cell proliferation marker (Ki-67) keratinocytes. The results assessed a semiquantitative manner. data analyzed statistical software package IBM SPSS 20. conclusions indicated that Ag/PVA/Gr might be used as wound dressings enhance healing potential established faster integration shorter retention tissue.